Abstract
Microbial protein is the most variable and uncertain element of current systems of evaluating protein requirements for ruminants. The variability is partly a result of the multiplicity of techniques used to quantify microbial nitrogen (MN) yield and partly due to unrepresentative sampling of microbial cells. In a number of studies in the literature it is tacitly assumed that marker:N ratios in rumen liquid associated bacteria (LAB), solid associated bacteria (SAB) and duodenal digesta bacteria (DDB) are identical. Under ordinary feeding conditions ruminal SAB are numerically predominant and may comprise 50-75% of the total microbiota (Mitsumori and Minato, 1997). This study highlights the variations in chemical composition in LAB, SAB and DDB and their implications in estimating microbial growth. The study also simultaneously compares four microbial markers in vivo.
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