Abstract
The influence of potassium sorbate, sodium benzoate, sulfur dioxide (SO2) and temperature on biomass and patulin production by Byssochlamys nivea in grape juice was investigated. Growth of B. nivea was monitored over a 25-d incubation period at 21, 30 and 37°C. Approximately 2,500 mg (dry weight) of biomass per 100 ml of juice was obtained in controls at 30 and 37°C; significantly lower amounts were observed at 21°C. Based on concentration, SO2 had the most significant effect on reducing biomass production followed by potassium sorbate and sodium benzoate, respectively. Patulin was produced in the highest concentrations (10 mg/100 ml) at 21°C after 20 d of incubation. Production was less at 30 and 37°C, with a fairly rapid decrease after reaching a maximum concentration. As in the biomass study, SO2 had the most significant influence on inhibiting patulin production followed by potassium sorbate and sodium benzoate.
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