Abstract
Recent studies have demonstrated the potential of in vitro Urena lobata L. hairy roots to inhibit α-glucosidase for supporting the treatment of type 2 diabetes. To increase the productivities of hairy roots with α-glucosidase inhibitory activity in in vitro culture conditions, this study focus on the effects of some metabolic factors such as precursors (L-phenylalanine, L-tyrosine) and elicitors (chitosan, methyl jasmonate, salicylic acid). They were added to the culture media to investigate the growth and α-glucosidase inhibitory activity of Urena lobata L. hairy roots. The results showed that for the effects of precursors, only phenylalanine (1 µM) increased root biomass with the highest of α-glucosidase inhibitory activity on the 25th day of culture. In contrast, tyrosine did not play any role in increasing the biomass and α-glucosidase inhibitory activity in Urena lobata L. hairy roots. For the effects of elicitors, only chitosan (50 mg/L) resulted in hairy roots with α-glucosidase inhibitory activity higher than the control after 3 days in culture medium. Other elicitors such as methyl jasmonate, salicylic acid had lower α-glucosidase inhibitory activity than the control. The results of this study demonstrated the potential of phenylalanine and chitosan in increasing the productivity of in vitro hairy roots with higher α-glucosidase inhibitory activity in Urena lobata L.
Highlights
To increase the productivities of hairy roots with α-glucosidase inhibitory activity in in vitro culture conditions, this study focus on the effects of some metabolic factors such as precursors (L-phenylalanine, L-tyrosine) and elicitors (chitosan, methyl jasmonate, salicylic acid)
The results showed that for the effects of precursors, only phenylalanine (1 μM) increased root biomass with the highest of α-glucosidase inhibitory activity on the 25th day of culture
The results of this study demonstrated the potential of phenylalanine and chitosan in increasing the productivity of in vitro hairy roots with higher α-glucosidase inhibitory activity in Urena lobata L
Summary
Các nghiên cứu gần đây đã chứng minh tiềm năng của rễ tơ in vitro Ké hoa đào (Urena lobata L.) trong việc ức chế α-glucosidase, có thể dùng trong hỗ trợ điều trị bệnh đái tháo đường tuýp 2. Các yếu tố biến dưỡng như tiền chất (L-phenylalanine, L-tyrosine) và elicitor (chitosan, methyl jasmonate, acid salicylic) sẽ được bổ sung vào môi trường nuôi cấy để khảo sát sự tăng trưởng và hoạt tính ức chế α-glucosidase của rễ tơ Ké hoa đào. Kết quả cho thấy đối với ảnh hưởng của tiền chất, chỉ có phenylalanine 1 μM làm gia tăng sinh khối rễ và rễ tơ có hoạt tính ức chế α-glucosidase cao nhất ở ngày nuôi cấy thứ 25. Đối với ảnh hưởng của các elicitor được khảo sát, chỉ có chitosan 50 mg/L sau 3 ngày cảm ứng trong môi trường nuôi cấy thì rễ tơ có hoạt tính ức chế α-glucosidase cao hơn mẫu đối chứng. Đây là bài báo công bố mở được phát hành theo các điều khoản của the Creative Commons Attribution 4.0 International license
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