Abstract
The possible utility of static culture conditions for sporulation studies was evaluated. The effects of a series of potential amino acid antagonists on the growth and sporulation of a strain of Bacillus cereus in a defined medium were compared under both static and shaken culture conditions. A randomly picked series of 24 analogues was observed to produce all of the possible effects (no inhibition of growth or sporulation, inhibition of growth, inhibition of sporulation, delay of growth and sporulation) with no important qualitative differences between the shaken and static systems. The commonly accepted premise that shaken cultures must be used for sporulation studies has no general validity; the simpler technique of static culture may have great value for observation of the developmental stages of sporulation.
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