Effects of sodium new houttuyfonate on proliferation, apoptosis and migration of A2780 cells

Abstract

Objective: To observe the effects of sodium new houttuyfonate on proliferation, apoptosis and migration of human epithelial ovarian cancer A2780 cells, and to investigate the inhibition of invasion and migration of human epithelial ovarian cancer A2780 cells by regulating the expression of apoptosis-related molecules. Methods: blank group and different dose groups (100, 200, 300,400 and 500 μg·ml-1of sodium new houttuyfonate) were treated with human ovarian cancer A2780 cells for 48 h, respectively. MTT assay was used to detect cell proliferation and activity, and flow cytometry was used to detect cell proliferation inhibition rate and apoptosis rate. The cell migration was observed and the healing rate and migration rate of scratch were calculated. The mRNA expression of apoptosis-related molecules Bcl-2, Bax, VEGF and NF-κBp65 was detected by real-time PCR. Results: MTT and flow cytometry results showed that compared with blank group, the cell inhibition rate of sodium neohouttuynium was significantly increased (P<0.01), and the total cell apoptosis rate was increased (P<0.05); The results of scratch test showed that compared with blank group, the migration ability of new houttuynia sodium group was weakened, and the number of cell migration was significantly reduced. PCR results showed that compared with the blank group, the mRNA expression of VEGF, Bcl-2 and NF-κBp65 in the group of neohouttuynia sodium decreased, and the expression of Bax increased. Conclusion: sodium new houttuyfonate can inhibit proliferation and promote apoptosis of human ovarian cancer A2780 cells, and may inhibit the migration of A2780 cells by regulating the expression of apoptosis-related molecules.