Effects of sodium aescinate on proliferation and apoptosis of human cholandiocarcinoma Hccc9810 cell line

Abstract

Objective To investigate the effects of sodium aescinate on the proliferation and apoptosis of cholangiocarcinoma Hccc9810 cell line.Methods The effects of sodium aescinate (0,10,20,40μmol/L) on cell growth,cycle and apoptosis were investigated by methyl thiazol tetrazolium (MTT) assay,flow cytometry,and fluorescence microscopy.Results Sodium aescinate inhibited cholangiocarcinoma cell growth in a dose- and time-dependent manner,and its half-inhibitory concentration (IC50) for 24 h,48 h,and 72 h was (45.34 ±2.12),(33.00± 1.92) and (25.00 ± 1.65) μmol/L respectively.The proportion of cells in G1 phase was increased from (49.49 ± 1.22)％ to (70.20 ± 1.52)％.The apoptosis rote was increased from 20.54％ to 62.56％ (P ＜0.05).Conclusion Sodium aescinate exhibited its anticarcinoma effect probably through regulating cell cycle and inducing cell apoptosis. Key words: Sodium aescinate; Proliferation; Cholangiocarcinoma