Effects of Small Red Bean Powder on Lipid Metabolism and Antioxidant Capacity in High Cholesterol and High Fat Diet Induced Nonalcoholic Fatty Liver Disease (NAFLD) Mice

Abstract

NAFLD is most frequently occurred liver disease caused by high cholesterol and high fat diet. NAFLD is highly related to metabolic disease like obesity, type 2 diabetes and dyslipidemia. In this study, we investigated the effects of small red bean powder on lipid metabolism and antioxidant capacity in NAFLD mice. Mice were randomly divided into 4 groups then fed different diets: normal diets(ND), high cholesterol and high fat diets(HD); and HD diets with 10%(10RB‐HD) and 20%(20RB‐HD) small red bean powder. Body weight gain for 4 groups was ND(5.55±1.27g), HD(15.11±2.00g), 10RB‐HD(8.90±1.17g) and 20RB‐HD(9.54±0.83g). Liver weight in HD(1.63±0.19g) was significantly higher than ND(1.02±0.04g) and decreased in 10RB‐HD(1.23±0.07g) and 20RB‐HD(1.31±0.12g). Abdominal and epididymis adipose tissue weights in all experimental groups were lower than that of HD(0.67±0.13g and 2.29±0.25g). Blood glucose level in 10RB‐HD and 20RB‐HD(156.7±33.78mg/dl, 127.57±31.45mg/dl) was significant lower than HD(274.57±44.76mg/dl). Serum triglyceride and total cholesterol concentration significantly decreased in 10RB‐HD(TG; 118.10±3.38mg/dl, TC; 124.57±8.21mg/dl) and 20RB‐HD(TG; 111.47±2.23mg/dl, TC; 116.51±8.97mg/dl) than HD(TG; 127.88±3.07mg/dl, TC; 201.83±26.19mg/dl). Liver glutathione peroxidase activity in all experimental groups (5.55±0.2104U/ml and 6.34±0.6104U/ml) significantly increased than HD(3.75±0.04U/ml). These results suggest that small red bean powder could improve lipid metabolism and antioxidant capacity, and be meaningful because there are not enough studies for small red bean related to NAFLD. Further lipid and antioxidant indexes are under analysis therefore, it could be more discussed.