Effects of sleep deprivation induced blood stasis syndrome on platelet activation in rats

Abstract

Blood stasis syndrome is the pre-state of thrombotic disease. The model of blood stasis syndrome in rats was induced by sleep deprivation to study on effects of blood stasis syndrome on platelet activation. The weight, the color of tongue and hemorheology for the blood stasis syndrome of Chinese medicine were measured after modeling. The release of platelet granules and platelet activation factors in plasma were detected by ELISA kit related indicators to provide experimental basis for platelet function evaluation and related drug effects in syndrome research. The results showed that the weight of the model group rats was significantly lower than that of the normal group (P<0.01). The tongue showed a dark purple blood stasis pattern, and the R, G and B values of the tongue surface in model group were significantly lower than those of the normal group (P<0.01). The hemorheological parameters including high shear, middle shear and low shear viscosity in whole blood were significantly higher than those in the normal group (P<0.01). But plasma viscosity did not change significantly. The release levels of platelet α particles (GMP-140, β-TG, PF4) and dense particles (ADP, 5-HT) were significantly higher than those in the normal group (P<0.01). The levels of TXB₂ and 6-keto-PGF₁α in plasma were significantly higher than those in the normal group (P<0.01). The ratios of TXB₂ and 6-keto-PGF₂α were also significantly higher than those in the normal group (P<0.01). The levels of PAF in plasma in model group were significantly higher than those in the normal group (P<0.01). It was concluded that platelet functions could be changed induced by sleep deprivationin rats with blood stasis syndrome, and there might be inflammation and endothelial cell dysfunction.