Abstract
Immobilized metal affinity chromatography (IMAC) is a popular technique used for large‐scale purification of proteins because of its high binding specificity, capacity and relative ease and cost of operation. Despite recent advances in improving the robustness of the technique, several factors still affect the level of purity and yield of proteins during IMAC. Using (His)6‐tagged proteins with comparably high level of expression, we are investigating the effects of factors such as protein size, number and position of tags, species of bivalent ions, buffer composition and commonly used additives on the purity and yield of purification in IMAC. Initial experimental data suggests that size of protein has significant influence on purity and yield of purification of (His)6‐tagged proteins using Ni2+ and Co2+ IMAC.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
