Effects of single non- ortho, mono- ortho, and di- ortho chlorinated biphenyls on cell functions and proliferation of the human prostatic carcinoma cell line, LNCaP

Abstract

This study investigated the effects of the non- ortho 3,3′,4,4′,5-pentachlorobiphenyl (PCB 126) and 3,3′,4,4′-tetrachlorobiphenyl (PCB 77), the mono- ortho 2,3′,4,4′,5-pentachlorobiphenyl (PCB 118), and the di- ortho substituted 2,2′,4,4′,5,5′-hexachlorobiphenyl (PCB 153) on the human prostatic carcinoma cell line LNCaP. Activities of 5α-reductase and ethoxyresorufin- O-deethylase (EROD) secretion of testosterone-regulated prostatic specific antigen (PSA) and cell proliferation served as specific and general cell markers. The two non- ortho (dioxin-like) PCBs, PCB 126 and PCB 77, showed anti-androgenic properties. Both PCBs reduced androgen-depending PSA secretion and cell proliferation, and inhibited the DHT-producing enzyme 5α-reductase in a concentration-dependent manner. In contrast, the ortho-substituted PCBs, PCB 118 and PCB 153, had no effect on 5α-reductase. However, they had a biphasic effect on LNCaP cell proliferation. PCB 153 and, to some extent, PCB 118 induced cell proliferation and PSA secretion at low concentrations, whereas, these parameters were reduced at high concentrations. Since EROD induction and inhibition of 5α-reductase activity was not observed, these findings suggest an AhR and AR independent mechanism and post-transcriptional target sites should be considered. The effects of PCB 126 on cell proliferation, PSA secretion, and 5α-reductase activity may be partly a result of AhR-dependent inhibition. In conclusion, the endocrine activity of PCBs via direct steroid hormone receptor-mediated and Ah receptor-mediated pathways are well known. However, the modulation of post-transcriptional targets may account for some of the endocrine disrupting properties of single PCB congeners.