Abstract
Epilepsy is one of the most common neurological disorders, characterized by recurrent seizures, which may increase the content of reactive oxygen and nitrogen species. The objective of this study was to investigate the effects of Neuropeptide Y on oxidative and nitrosative balance and brain-derived neurotrophic factor levels induced by pentylenetetrazole (a standard convulsant drug) in the hippocampus of Wistar rats. Three groups of seven rats were treated intraperitoneally as follows: group 1 (saline + saline) 1 ml saline, group 2 (salin + Pentylenetetrazole) 1 ml saline 30 min before Pentylenetetrazole; and group 3 (Neuropeptide Y + Pentylenetetrazole) 60 μg/kg Neuropeptide Y 30 min before 60 mg/kg Pentylenetetrazole. After 24 h, the animals were euthanized by decapitation. Hippocampus were isolated to evaluate the malondialdehyde, glutathione, nitric oxide, and brain-derived neurotrophic factor levels in three rat groups. The results of this study demonstrated that while intraperitoneally administered neuropeptide Y did not result in a statistically significant difference in BDNF levels, its administration caused a statistically significant decrease in malondialdehyde and nitric oxide levels and an increase in glutathione levels in rats with pentylenetetrazole-induced epileptic seizure. Neuropeptide Y were able to reduce nitroxidative damage induced by pentylenetetrazole in the hippocampus of Wistar rats.
Highlights
Generalized epilepsy is a chronic disorder characterized by recurrent seizures, which has been shown to cause an increase in reactive oxygen species (ROS) in the brain and increased oxidative stress [ ]
Hippocampal Nitric oxide (NO) levels were significantly higher in the SF + PTZ group than in the SF + SF and SF + Neuropeptide Y (NPY) groups ( . ± . , . ± . , . ± μmol/ mg protein, p < . , respectively) (Figure )
In contrast the mean GSH levels were significantly lower in the SF + PTZ group than in the SF + SF, and SF + NPY
Summary
Generalized epilepsy is a chronic disorder characterized by recurrent seizures, which has been shown to cause an increase in reactive oxygen species (ROS) in the brain and increased oxidative stress [ ]. There is increasing evidence that the peptide exerts powerful anticonvulsant activities and a related neuroprotective effect on cornu ammonis (CA) pyramidal neurons [ , , ]. For these reasons, we studied the acute effects of exogenous intraperitoneally (ip) administered NPY in adult rats following pentylenetetrazole (PTZ) intoxication, during the early phases that are reasonably crucial in the establishment of brain injury.
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