Abstract
The paper presents the study results of silver nanoparticles (AgNPs) effectiveness for woody species explants sterilization as exemplified by hairy birch (Betula pubescens) at various stages of micro-clone propagation. It was shown that simultaneous application of 0.03% AgNPs+15% NaOCl at the stage of explant sterilization resulted in consistently sterile (90%) cell cultures throughout the 28-days cultivation period. When the growth medium was modified with AgNPs at concentrations of 1.5 - 3 μg/l at the multiplication and rooting stages it dramatically reduced phytopathogenic contamination of the explants and regenerants thus increasing the number of surviving plantlets up to 100%. Besides, the analysis results carried out for the photosynthetic and enzyme activity display high stress-resistance potential of the studied plants compared with the control. The obtained results allow one to conclude that AgNPs have high effectiveness and application potential when used in microclonal propagation of woody plants.
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