Abstract
Silver nanomaterials have been mainly developed as antibacterial healthcare products worldwide, because of their antibacterial activity. However, there is little data regarding the potential risks and effects of large amounts of silver nanomaterials on plants. In contrast, N-glycans play important roles in various biological phenomena, and their structures and expressions are sensitive to ambient environmental changes. Therefore, to assesse the effects of silver nanomaterials, we focused on the correlation between N-glycans and the effects of silver nanomaterials in plants and analyzed N-glycan structures in Oryza sativa seedlings exposed to silver nanocolloids (SNCs). The phenotype analysis showed that the shoot was not affected by any SNC concentrations, whereas the high SNC exposed root was seriously damaged. Therefore, we performed comparative N-glycan analysis of roots. As a result, five of total N-glycans were significantly increased in SNC exposed roots, of which one was a free-N-glycan with one beta-N-acetylglucosamine residue at the reducing end. Our results suggest that the transition of plant complex type N-glycans, including free-N-glycans, was caused by abnormalities in O. sativa development, and free-N-glycan itself has an important role in plant development. This study originally adapted glycome transition analysis to environmental toxicology and proposed a new category called “Environmental glycobiology”.
Highlights
Nanomaterial is a general term for small substances that are 1–100 nm in diameter
High-mannose type N-glycans are synthesized in endoplasmic reticulum (ER), and other type N-glycans are synthesized in the Golgi apparatus
To assesse the effects of silver nanomaterials, we focused on the correlation of N-glycan structures and the effect of silver nanomaterials in Oryza sativa and analyzed the N-glycan structures in silver nanocolloids (SNCs) exposed O. sativa seedlings
Summary
Risa Horiuchi[1], Yukari Nakajima[2], Shosaku Kashiwada1,3 & Nobumitsu Miyanishi[1,2,3,4]. To assesse the effects of silver nanomaterials, we focused on the correlation between N-glycans and the effects of silver nanomaterials in plants and analyzed N-glycan structures in Oryza sativa seedlings exposed to silver nanocolloids (SNCs). The toxicity of silver nanoparticles affects the expression of several proteins that are mainly involved in primary metabolism and cell defense in wheat seedlings[9]. The above-mentioned studies showed that silver nanomaterials have high toxicity in plants. Many N-glycan structures are linked to proteins or peptides and are closely involved in all life phenomena, such as development, signaling, 1Graduate School of Life Sciences, Toyo University, Gunma, 374-0193, Japan. N-glycan structural analysis is valuable for the risk assessment of silver nanomaterial toxicity in plants. To assesse the effects of silver nanomaterials, we focused on the correlation of N-glycan structures and the effect of silver nanomaterials in Oryza sativa and analyzed the N-glycan structures in SNC exposed O. sativa seedlings
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
