Effects of silicon on copper toxicity in Erica andevalensis Cabezudo and Rivera: a potential species to remediate contaminated soils

Abstract

The influence of silicon on responses to copper excess was studied in plants of Erica andevalensis. Plantlets were grown in nutrient solutions containing two Cu (1 and 500 µM) and three Si concentrations (0, 0.5 and 1 mM). Plant growth, water content, and mineral nutrient concentration were determined. Plants grown with 500 µM Cu showed differences in growth and shoot water content depending on Si supply. The addition of 1 mM Si in high-Cu nutrient solutions significantly improved plant growth and reduced water loss preventing plant death related to Cu-excess. Silicon supply reduced significantly leaf Cu concentration (up to 32%) and increased Cu concentration in roots. Phytoliths isolated from leaves were analysed by scanning electron microscopy coupled with energy-dispersive X-ray spectroscopy. Such phytoliths consisted in silica deposits associated with Cu and other elements (K, Ca, P). Improvement by Si of Cu tolerance in E. andevalensis was clearly related to the inhibition of Cu upward transport. The leaf phytoliths formed in Si-treated plants might have some contribution to tolerance by Cu immobilisation and inactivation.