Abstract
Introduction. Analysis of canine lipoprotein fractions after agarose gel electrophoresis (agEF) separation could be an important diagnostic tool in primary and secondary dyslipidemia diagnosis. The aim of this study was to measure concentrations of triglycerides and cholesterol and to analyze lipoprotein fractions in dogs after basal (12 hours) and short-term (24 and 36 hours) fasting, i.e., frequent conditions in clinical practice. Materials and Methods. Blood samples were collected from six lean dogs of both sexes and different breeds, after 12, 24 and 36 hours of fasting. Concentrations of glucose, triglycerides and cholesterol were determined on an automated wet biochemistry analyzer, lipoprotein fractions were separated by agEF and leukocyte numbers were assessed on an automated hematology analyzer. Results and Conclusions. Results showed there was no significant change in glucose, triglyceride and cholesterol concentrations nor in leukocyte numbers during dog fasting. Moreover, there was no change in ?1- and ?2-fractions, but there was a significant decrease in pre?- and ?-fraction of lipoproteins. It is know that high density lipoproteins (HDL) have ?-mobility and very low density (VLDL) and low density lipoproteins (LDL) have pre?- and ?-mobility. Thus, it is possible that reverse cholesterol transport maintained by HDL is not affected during short-term fasting. On the contrary, synthesis of VLDL and formation of LDL are probably decreased because endogenous synthesis of triglycerides is decreased or their clearance is increased.
Highlights
Analysis of canine lipoprotein fractions after agarose gel electrophoresis separation could be an important diagnostic tool in primary and secondary dyslipidemia diagnosis
Veterinarski Glasnik 2018, 72 (1), 35-43 of very low density Lps (VLDL) and formation of low density Lps (LDL) are probably decreased because endogenous synthesis of triglycerides is decreased or their clearance is increased
Our results demonstrated that total cholesterol, triglyceride and glucose concentrations did not change significantly during fasting (Figure 1)
Summary
Analysis of canine lipoprotein fractions after agarose gel electrophoresis (agEF) separation could be an important diagnostic tool in primary and secondary dyslipidemia diagnosis. Lipoproteins (Lps) are a heterogeneous group of molecules involved in lipid transport between tissues. According to their hydrated density, structure, and function, Lps are divided into chylomicrons (CMs), very low density Lps (VLDL), low density Lps (LDL) and high density Lps (HDL). One of the fast and easy methods to visualize serum Lps is agarose gel electrophoresis (agEF). Fractions that can be obtained after agEF of canine serum are: α1-, α2-, preβ- and β-fractions, considered to be HDL-2, HDL-1, VLDL and LDL, respectively (Mahley et al, 1974). The utility of canine Lp fraction analysis in clinical and research settings is not fully investigated
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