Effects of sheep bone biochar on soil quality, maize growth, and fractionation and phytoavailability of Cd and Zn in a mining-contaminated soil

Abstract

Biochar prepared from various feedstock materials has been utilized in recent years as a potential stabilizing agent for heavy metals in smelter-contaminated soils. However, the effectiveness of animal bone-derived biochar and its potential for the stabilization of contaminants remains unclear. In the present study, sheep bone-derived biochar (SB) was prepared at low (500 °C; SBL) and high temperatures (800 °C; SBH) and amended a smelter-contaminated soil at 2, 5, and 10% (w/w). The effects of SB on soil properties, bioavailable Zn and Cd and their geochemical fractions, bacterial community composition and activity, and the response of plant attributes (pigments and antioxidant activity) were assessed. Results showed that the SBH added at 10% (SBH10) increased soil organic carbon, total nitrogen, and phosphorus, and also increased the oxidizable and residual Zn and Cd fractions at the expense of the bioavailable fractions. The SBH10 lowered the Zn and Cd contents in maize roots (by 57 and 60%) and shoot (by 42 and 61%), respectively, compared to unamended control. Additionally, SBH10 enhanced urease (98%) and phosphates (107%) activities, but reduced dehydrogenase (58%) and β-glucosidase (30%) activities. Regarding the effect of the pyrolysis temperature, SBH enhanced the activity of Acidobacteria, Bacteroidetes, Firmicutes, Nitrospirae, Verrucomicrobia, Chlorobi, and Microgenomates, but reduced Actinobacteria and Parcubacteria in comparison to SBL. However, only the SBL10 reduced the Proteobacteria community (by 9%). In conclusion, SB immobilized Zn and Cd in smelter-affected soils, enhanced the bacterial abundance and microbial function (urease, phosphates), and improved plant growth. However, validation of the results, obtained from the pot experiment, under field conditions is suggested.