Abstract

The principles of colorimetric detecting oligonucleotides with the help of gold nanospheres and nanorods are discussed. Marker sequences of fragments of HIV-1 genome and Bacillus anthracis are used as models. Experimental data are reported that demonstrate the influence of gold nanorod morphology on the reproducibility of colorimetric tests. A new method is proposed for detecting oligonucleotides based on the application of positively charged gold nanospheres in combination with absorption spectroscopy and dynamic light scattering. Charge reversal of negatively charged gold nanospheres is implemented through the bilayer adsorption of cetyltrimethylammonium bromide molecules. The sensitivity of the proposed method is comparable with the detection of DNA sequences via the colorimetric protocol using nanorods, but it is more simple and stable from the viewpoint of realization. It is shown that the colorimetric tests using gold nanorods and nanospheres do not provide reliable information on the presence of single- and three-base mismatches in target oligonucleotides.

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call

Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.