Effects of shah mette lo/fluticasone propionate on airway inflammation in a rat model with COPD

Abstract

Objective To investigate the effects of mette lo (50 μg)/fluticasone propionate (250 μg) on airway inflammation in a rat model with chronic obstructive pulmonary disease(COPD).Methods Thirty rats were randomly divided into three groups.Group A received normal breeding as controls.Group B and group C received LPS (200 μg,intratracheally injected at the 1st and the 14th day) and tobacco exposure (from the 2nd day to the 28th day except the 14th day) to establish COPD model,and group C received a nebulized dose of mette lo (50 μg)/fluticasone propionate (250μg) 30 minutes before the tobacco exposure each time.Airway resistance and compliance were measured before sacrificed.Histological examination was performed with Hematoxylin-Eosin staining.The differential cells counts in bronchoalveolar lavage fluid (BALF) were examined.The concentrations of IL-8,TNF-α and MMP-9 in blood and BALF were determined by ELISA.Results Airway resistance significantly increased (z =2.72,P ＜0.05) and pulmonary dynamic compliance significantly reduced (z =-1.97,P ＜0.05) in rats in the group B compared with those in the group A,and airway resistance significantly reduced(z =-2.42,P ＜0.05) and pulmonary dynamic compliance significantly increased(z =2.19,P ＜0.05) in rats in the group C compared with those in the group B (P ＜0.05).Severe lung inflammation and emphysema were demonstrated in rats in group B,and the pathological changes in rats in group C were mild.The total cell,neutrophils and macrophage in BALF were significantly higher in group B than those in group A (z =3.79,3.79,3.78,P ＜0.05),and the inflammatory cells in BALF were significantly lower in group C than those in group B (z =-3.78,-3.78,-3.78,P ＜0.05).The level of IL-8,TNF-a in serum and BALF (t =3.72,3.33,5.01,2.45,P ＜0.05) and MMP-9 in serum and BALF (z =2.04,2.50,P ＜0.05) were significantly higher in group B than those in group A,and the level of IL-8,TNF-α in serum and BALF (t =-3.54,-2.64,-4.11,-2.64,P ＜0.05) and MMP-9 in serum and BALF(-2.27,-2.38,P ＜ 0.05) were significantly lower in group C than those in group B.Conclusions Mette lo (50 μg)/ fluticasone propionate (250 μg) can alleviate the lung inflammation by inhibiting the activities of inflammatory cells and release of inflammatory mediators,and can prevent airway remodeling by inhibiting MMP-9. Key words: Chronic obstructive pulmonary disease; Mette lo (50 μg)/fluticasone propionate (250 μg) ; Airway inflammation; Interleukin-8 ; Tumor necrosis factor-α; Matrix memlloproteinase-9