Effects of sexual experience on conspecific odor preference and estrous odor-induced activation of the vomeronasal projection pathway and the nucleus accumbens in male rats

Abstract

Male rats prefer odors from estrous females to those from sexually active males. Several studies, however, have demonstrated that prior sexual experience was required to develop the preference for estrous odor. Immunohistological methods for visualizing Fos protein have been shown that in sexually experienced male rats, estrous odors activate brain areas throughout the vomeronasal projection pathway (VN pathway) and the nucleus accumbens (NAcc). In the present study, we examined the contribution of prior sexual experience to the estrous odor-induced neuronal activation of these brain areas in relation to the development of the preference for estrous odor. Sexually experienced testosterone-implanted castrates showed the preference for the odor from an estrous female as opposed to the odor from a sexually active male. In these subjects, significant increment of Fos-like immunoreactivity (Fos-Li) after exposure to estrous female soiled bedding was observed in all brain regions examined, confirming the results of previous studies. Sexually naïve subjects, on the other hand, did not show the preference for estrous odor and the significant increment of Fos-Li was observed only in the accessory olfactory bulb (AOB) and the posterior–dorsal medial amygdala (MePD) of the VN pathway. These results suggested that sexual experience is required for the estrous odor-induced activation of more central portions of the VN pathway, such as the medial preoptic area (mPOA) and the bed nucleus of the stria terminalis (BNST), and the NAcc. The activation of some of these brain regions, therefore, is probably involved in the development of the preference for estrous odor.