Effects of sevoflurane preconditioning on p-IκBα in a rat model of myocardial ischemia-reperfusion

Abstract

Objective To observe the effect of sevoflurane preconditioning on the expression of p-IκBα in a rat model of myocardial ischemia-reperfusion (I/R). Methods Eighty -four male SD rats after setting up the model of I/R, randomly were divided into seven groups: ①Control group; ② Simple-Ischemic group received 30 min I/R merely; ③ SEVO group inhaled 1.0 MAC sevoflurane for 30 min and 15 min wash-out followed by a 30 min I/R; ④ SEVO 165' group inhaled 1.0 MAC sevoflurane for 30 min and then stoped for 165 min; ⑤ PTN group received PTN 500 μg/kg(NF-κB inhibitor) intraperitoneally 60 min before I/R; ⑥ PTN±SEVO group and⑦SEVO+PTN group received PTN 500 μg/kg 15 min before and after sevoflurane preconditioning. Myocardium sample of all groups were collected before and after the time of I/R or the corresponding point in time. p- IκBα was determined by Western Blotting. Results The expression of p- IκBαwas significantly up-regulated in SEVO group(22±3)and SEVO 165'group(20±4) than that in Control group (15±3) before I/R(P<0.05); After perfusion the expression of p- IκBαin Simple-Ischemic group(44±6)and SEVO group (30±3) was significantly up-regulated than that in Control group( 15±4,P<0.05);but the up-regulated adjustment of SEVO group was smaller than that in Simple-Ischemic group (P<0.05). Conclusion This study further confirmed NF-κB participates in the I/R injury and it may play an important role in the mechanism of sevoflurane-induced cardioprotection. Key words: Anaesthetic,Inhalation; Myocardial reperfusion injury; Preconditioning; p-IκBα