Effects of sevoflurane on electroencephalography in immature rat brain

Abstract

Objective To investigate the effects of sevoflurane on neonatal rats electrocephalography(EEG)and the mechanism. Methods Thirty neonatal rats, aged 14 days, weighing 35- 45 g, were randomly divided into 3 groups: without sevoflurane group(group C,n= 10), low sevoflurane concentration group(group S1,n= 10), high sevoflurane concentration group(group S2). Electroencephalogram recording started immediately after completion of the implant surgery. Anesthesia was induced with 6.0% sevoflurane over 3 min and maintained with 2.1% over 60 min in group S1. In group S2 anesthesia was induced with 8.0% sevoflurane and maintained with 3.5%. Acquisition of the EEG was performed using the Sirenia software. Sirenia Score programs were used for the EEG data analysis. Results As compared with group D and group S1, spike's frequency and amplitude were significantly increased in group S2 during anesthesia induction. Spike's frequency was higher in group S2 than group D during anesthesia maintenaed. The concentration of corticosterone among three groups were:26.8±3.8, 106.5±15.1 and 155.9±13.6, respectively. Compared with group D, the level of corticosterone were significantly increased in group S1 and group S2. Conclusion Sevoflurane anesthesia can induce spike EEG in 14- days rats, which may be induced by high level of corticosterone. Key words: Sevoflurane; Immature rat; Electrocephgraphy; Spike