Effects of sevoflurane exposure on apoptosis and cell cycle of peripheral blood lymphocytes, and immunologic function

Zhimin Ji,Junfang Hu,Wanjun Wu,Li Li,Xinguo Wang,Weibin Yang,Cheng Zhang,Xueyong Peng,Fan Zhou,Qiuping Xu

doi:10.1186/s12871-021-01305-w

Abstract

BackgroundWaste anesthetic gases (WAGs) leaked from new-type halogenated inhalational anesthetics such as sevoflurane have been were reported to pose a risk for the health of operating room personnel. The effects of WAGs on peripheral blood lymphocytes, however, remain yet controversial. The present study was undertaken to examine the effects of occupational sevoflurane exposure on the peripheral blood lymphocytes of medical personnel who work in the operating room.MethodsA cohort of 56 medical residents were divided into exposed group (n = 28) and control group (non-exposed group) (n = 28). Gas chromatography was used to measure the concentration of sevoflurane in the medical resident’s breathing zone during surgeries under inhalation anesthesia in the exposure group. The gas collection lasted an hour. Peripheral blood lymphocytes were isolated from venous blood, and then apoptosis and cell cycle were analyzed by flow cytometry. EDTA-anticoagulated whole blood was harvested to analyze the lymphocyte subsets by flow cytometry. Immunoglobulins (IgA, IgM, IgG) were quantified by immunoturbidimetry.ResultsThe average concentration of sevoflurane in the exposed group was 1.03 ppm with a range from 0.03 ppm to 2.24 ppm. No significant effects were found on the apoptosis rates or cell cycles of peripheral blood lymphocytes in the exposed group relative to the control group (P > 0.05). Similarly, there were no significant differences in the lymphocyte subsets or the levels of immunoglobulins (IgA, IgM, IgG) between the two groups (P > 0.05).ConclusionsOccupational exposure to low-level sevoflurane has no significant effect on the peripheral blood lymphocytes of operating room staff, but this conclusion needs to be confirmed by multicenter and long-term follow-up studies with large samples.Trial registration number and date of registrationChiCTR2000040772, December 9, 2020 (Retrospective registration).

Highlights

Waste anesthetic gases (WAGs) leaked from new-type halogenated inhalational anesthetics such as sevoflurane have been were reported to pose a risk for the health of operating room personnel
Concentration of sevoflurane in exposed group is lower than the standard of National Institute for Occupational Safety and Health (NIOSH) After analyzing the concentration of sevoflurane collected from the breathing zone of all residents in the exposed group, we found that the average concentration of sevoflurane was 1.03 ppm with a range from 0.03 ppm to 2.24 ppm
Sevoflurane exposure has no significant effect on apoptosis and cell cycle of peripheral blood lymphocytes As shown in Table 2; Figs. 1, 2 and 3, we found that there were no statistically significant differences in the apoptosis rates or cell cycles of the peripheral blood lymphocytes between control group and exposed group (P > 0.05)

Summary

Introduction

Waste anesthetic gases (WAGs) leaked from new-type halogenated inhalational anesthetics such as sevoflurane have been were reported to pose a risk for the health of operating room personnel. The effects of WAGs on peripheral blood lymphocytes, remain yet controversial. The present study was undertaken to examine the effects of occupational sevoflurane exposure on the peripheral blood lymphocytes of medical personnel who work in the operating room. Mean DNA content of G1 control 28 48.54 2.46 1.62 0.11 phase. Mean DNA content of G2 control 28 97.41 5.98 1.83 0.07 phase occupational exposure to sevoflurane has harmful effects on the peripheral blood lymphocytes of medical personnel exposed to inhalational anesthetics in vivo

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