Effects of severe dehydration on leaf photosynthesis in Quercus petraea (Matt.) Liebl.: photosystem II efficiency, photochemical and nonphotochemical fluorescence quenching and electrolyte leakage.

Abstract

Leaf disks of oak (Quercus petraea (Matt.) Liebl.) trees were subjected to rapid dehydration in air in the dark. Optimal photochemical efficiency of PS II (F(v)/F(M)), photochemical (q(P)) and nonphotochemical (q(NP)) quenchings of chlorophyll a fluorescence, and relative conductivity (C(r)) of leaf disk diffusate were measured in leaf disks with different water deficits (D). No effect of dehydration was detected before D reached values above 0.30. When D increased from 0.30 to 0.50, q(NP) increased without any change in q(P), which may indicate that thermal deexcitation of PS II increased, allowing reduced photochemical activity and maintenance of a large pool of oxidized primary acceptors (QA), although carbon reduction was impaired. Large changes in electron transport chain activity, leading to decreases in both q(P) and q(NP), appeared only in leaf disks subjected to severe water deficits (D > 0.60) and were correlated with a modification of membrane structure. However, stability of F(v)/F(M) indicated that the functional integrity of PS II was not altered until D reached values above 0.75. We conclude that the photosynthetic apparatus of Q. petraea is rather insensitive to leaf dehydration per se during drought under natural conditions.