Abstract
ABSTRACT Serum from intact and from hypophysectomized adult male rats induces an ovarian ascorbic acid depletion in intact immature pseudopregnant recipients. When the recipients are in addition hypophysectomized, only the effect obtained with hypophysectomized donor serum is found, while normal serum is inactive. Similar results are seen when, instead of serum, hypothalamic tissue extracts are injected. Normal serum and hypothalamic tissue apparently act by way of the recipients' pituitary gland, and hence, by LH-RF. The effect produced by hypophysectomized donor material in hypophysectomized recipients is probably non-specific. In parallel experiments it was found that not all depleting activity is lost by heating adenohypophyseal extracts for 15 minutes in a boiling water-bath.
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