Effects of self‐pollination and outcrossing with cultivated plants in small natural populations of American ginseng, Panax quinquefolius (Araliaceae)

Abstract

For rare plants, self-pollination and inbreeding can increase in small populations, while unusual levels of outcrossing can occur through restoration efforts. To study both inbreeding and outcrossing, we performed experimental pollinations using Panax quinquefolius (American ginseng), a wild-harvested plant with a mixed mating system. For inbreeding, plants were either cross-pollinated within the population or self-pollinated, which resulted in a higher proportion of seeds from self-pollinated flowers. For outcrossing, wild plants were either cross-pollinated within the population or with cultivated plants from West Virginia or Wisconsin. Offspring of all crosses were followed for 4 yr. Two-yr-old seedlings from self-pollination had 45% smaller leaf areas and 33% smaller heights relative to those from cross-pollination. Leaf area is a positive predictor of longer-term survival in wild populations. Our results suggest inbreeding depression, which is unexpected in this self-fertile species. Seedlings from crosses with cultivated plants had 127% greater leaf area and 165% greater root biomass relative to outcrosses within the population. The accelerated growth suggests genetic differences between wild and cultivated populations, but outbreeding depression may not appear until later generations. Assessment of the ultimate fitness consequences of introducing cultivated genotypes requires monitoring over longer time periods.