Effects of selenium on benthic macroinvertebrates and fathead minnow (Pimephales promelas) in a boreal lake ecosystem

Abstract

Selenium (Se) is a contaminant of concern in many aquatic ecosystems due to its narrow range between essentiality and toxicity in oviparous (yolk-bearing) vertebrates. The objective of the present study was to determine the effects of Se, experimentally added to in situ limnocorrals as selenite, on invertebrate communities and fathead minnow (Pimephales promelas) at environmentally realistic Se concentrations. Nine limnocorrals were deployed in a mesotrophic lake at the International Institute for Sustainable Development – Experimental Lakes Area in Ontario, Canada in May 2017. From June 1 to August 17, 2017, selenite was added to six enclosures to attain mean measured aqueous Se concentrations of 1.0 ± 0.10 or 8.9 ± 2.7 μg/L Se (in triplicate) and three limnocorrals were untreated controls (background mean aqueous Se = 0.12 ± 0.03 μg/L). Benthic macroinvertebrates were collected throughout and at the end of the exposure period using artificial substrates to determine density, dry biomass, diversity, and taxa richness at the family level. Reproductively mature female fathead minnows (added on d 33 of the study) were collected throughout and at the end of the exposure period. After 77 d, Chironomidae and Gammaridae densities and biomass were significantly lower in the 8.9 μg/L Se treatment relative to the 1.0 μg/L Se treatment and the control. Invertebrate diversity (measured as Shannon’s and Simpson’s indices) significantly declined in the 1.0 μg/L and 8.9 μg/L Se treatments relative to the control (0.12 μg/L Se group). Fulton’s condition factor for fathead minnow was significantly less in the 8.9 μg/L treatment compared to 0.12 and 1.0 μg/L Se experimental groups. The results of this study indicated that exposure to relatively low aqueous selenite concentrations can negatively affect invertebrate density and biomass, as well as fish condition. More research is necessary to characterize the risk of selenite exposure to aquatic invertebrates under realistic field conditions, and future risk assessments may need to consider reduced food availability as a factor that may impair the health of higher trophic level organisms in areas with elevated selenite.