Effects of Seed Soaking with La(NO3)3 on Anti-oxidization System of Soybean Roots

Abstract

Rare earth (RE) is extensively applied in China, especially in agriculture. This experiment investigated the effects of seed soaking with lanthanum nitrate (La-0(CK), La-50(50mg· L-1), La-100(100 mg· L-1) and La-150(150 mg· L-1)) on reactive oxygen species, anti-oxidative substrates, anti-oxidative enzymes in soybean roots and its yield. The results showed that compared with other treatments, La concentration at 100mg· L-1 decreased the superoxide anion production rate, and hydrogen peroxide content, and increased the activities of superoxide dism utase(SOD), peroxidase (POD), and the contents of Ascorbic acid (AsA), Glutathione(GSH) in soybean roots during the whole growth stage. Besides, the yield of soybean had the same trend. The results revealed that metabolism rates of reactive oxygen species are restrained with the antioxidant ability of anti-oxidative system strengthened with the increase of La concentration, which presents protective effect on soybean plant. However, when the La concentration is higher than some concentration with around 150 mg· L-1, the activities of anti-oxidative enzymes and anti-oxidative substrates reduce resulting in reactive oxygen species accumulating, so the yield declines, which represents injured effects on soybean.