Abstract
Cotton seed factors, including seed-surface pH, cultivar, and presence of a seed-treatment fungicide, were investigated for their effect on spermosphere and rhizosphere colonization by the commercial biological control agent Bacillus subtilis strain GB03 (GB03). Commercial (nonneutralized) cotton seed is traditionally acid-delinted and in these studies was found to have surface pH values ranging from 1.90 to 3.50. Following seed treatment with GB03, spermosphere populations of GB03 were up to 5-10-fold lower on nonneutralized (commercial acid-delinted) cotton seed than on neutralized seed grown in sterile sand or in field soil, respectively; however, rhizosphere populations of GB03 on 2-wk-old cotton root systems did not differ significantly [...]
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