Abstract
All eukaryotes universally contain large amounts (20-30%) of higher sterols in their plasma membranes. It remains a mystery why different eukaryotic kingdoms have chosen different higher sterols for their membrane reinforcement, such as cholesterol in animals, ergosterol in fungi, phytosterols in plants, and e.g. desmosterol and fucosterol in algae. We have used a range of biophysical techniques, including calorimetry, fluorescence microscopy, atomic-force microscopy, and vesicle-fluctuation analysis, to assess the various physical effects of fucosterol on lipid membranes.
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