Abstract

Pure culture sclerotia of Sclerotium cepivorum stimulated development of a number of bacteria on agar media. Using Bacillus subtilis and a chemically defined medium it was found that sclerotia could compensate for the absence of a carbon source but not of other constituents. The ethanol soluble carbohydrates of sclerotia grown on a number of carbon sources were identical to those produced on onion bulbs. Using 14C-labelled sclerotia it was found that the major substances exuded in agar, and in water, were trehalose, glucose and mannitol. Small quantities of a glucan were also present in sclerotial exudates. Surface sterilization with calcium hypochlorite increased the degree of exudation by sclerotia. No evidence was obtained to indicate long-term exudation by sclerotia in soil.

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