Effects of schizophrenic cerebrospinal fluid on macaque's behaviors

Abstract

Objective To investigate whether there were behavioral changes of macaque after being injected cerebrospinal fluid (CSF) of schizophrenics in their cerebrospinal cavities. Methods By random selection strategy,according to the cerebral Computer Tomography (CT) presentations, CSF of schizophrenics with abnormal brain structures, schizophrenics with normal brain structures, healthy volunteers, and normal saline were separately injected into macaque' s cerebrospinal cavities via lumbar puncture. According to the inoculated matter, macaque were divided in sequence into case group A (CGA) ,case group B (CGB) ,healthy control group(HCG) and normal saline group(NSG). Behavioral scale made by investigators themselves was used for assessing macaque's behaviors for 3 weeks as the basis data before inoculation and for 12 weeks after that. Data of basis,the 2nd,4th, 6th,8th, 10th, 12th weekend after treatments were used in statistic analysis. Results After treatments the total behavior scale scores of CGA (31.00±3.83) and CGB (28.20±4.92) were higher than that before treatments (21.67±1.94,22.33±0.47 respectively). HCG got lower total behavior scale score after treatment (17.00± 4.24) than before (22.17±0.24). The total behavior scale score of NSG after treatment was not different from it before treatment. Two case groups have higher total scores of behavior scale than HCG and NSG at 6 time point after treat. General linear model analysis show treatment * time intercept effect (F=21.67, P<0.01) were statisticaUy significant. Scores of 8 behavioral factors at every time points after treatments were analyzed by Wilcoxon test. The results show all two case groups had higher scores of aggressiveness (P=0.046) ,general activities (P =0.039), feistiness (P=0.026) than HCG and NSG, and these differences got more significant with time. Condusiou Injecting schizophrenic CSF into macaque's cerebrospinal cavities may result in significant behavioral changes of macaque, such as more aggressive ,activating and feisty. Key words: Schizophrenia; Cerebrospinal fluid; Macaque; Behavior