Abstract
Abstract Objectives To investigate the influence of preservation methods and processes on the plasma interleukin-6 (IL-6) stability. Methods Lithium-heparin plasma was collected from female patients: 32 female patients with invasive breast neoplasms and 20 healthy females. Each sample was divided into three tubes. Samples were stored at different temperatures or at different times. The concentration of IL-6 was detected. Results IL-6 levels in patients were not altered significantly compared to the control group after storage at 4 °C or 25 °C for 12 h. However, IL-6 levels were significantly higher compared to controls (p<0.05) after storage at 25 °C for 48 h. IL-6 levels in patients with breast neoplasms were significantly higher compared to the control group (p<0.05) when stored at 4 °C after 12 h. IL-6 levels in patients with breast neoplasm increased more than 10-folds compared to the control group after only 2 h storage at 25 °C. Conclusions Concentrations of IL-6 in breast neoplasms samples significantly change under different storage conditions. Pretreatment needs to be standardized for blood sample handling procedure. Comparison of different storage conditions of IL-6 levels may not be reliable.
Highlights
Breast cancer is the second leading cause of cancer death among women [1]
IL-6 is considered as a multifunctional cytokine with obviously tumor-promoting effects in breast neoplasms
Stage 1–4 breast neoplasms cancer patients plasma were collected. These patients were composed of 30 women with breast neoplasms, ranging between 28 and 58 years
Summary
Breast cancer is the second leading cause of cancer death among women [1]. Diagnosis and treatment can greatly improve outcomes. Inflammation is often associated with the development and progression of breast cancer [2]. IL-6 is produced by a variety of cell types, including macrophages, T cells, B cells, endothelial cells, and tumor cells [3]. IL-6 plays an important role in immune-related diseases, such as tumors [4]. IL-6 plays an important role in breast cancer for its important role in immunity [5]. IL-6 is considered as a multifunctional cytokine with obviously tumor-promoting effects in breast neoplasms. High IL-6 levels are correlated with poor overall survival in metastatic breast neoplasm
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