Effects of Salt Stress on Cell Surface Properties and Symbiotic Performance of Root Nodulating Bacteria

Ashok Kumar Singh Ashok Kumar Singh,Rajendra Prasad Bhatt Rajendra Prasad Bhatt,Gauri Singh Gauri Singh

doi:10.20510/ukjpb/3/i1/89222

Ashok Kumar Singh Ashok Kumar Singh, Rajendra Prasad Bhatt Rajendra Prasad Bhatt + Show 1 more

Open Access

https://doi.org/10.20510/ukjpb/3/i1/89222

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Abstract

In the current study fifty root nodulating bacterial strains isolated from Cicer arietinum (Chickpea) and Vigna radiata (Mungbean) nodules were subjected to different NaCl concentrations. Out of these 20 salt tolerant isolates were selected for the production of cell surface component like β-glucan, lipopolysaccharide, exopolysaccharide and motility under salt stress condition. Symbiotic performance of Vigna radiata and Cicer arietinum were done under salt stress condition by using 4 isolates (CMR3,CMR13,MR5.MR11) which showed the tolerant behavior in all cell surface properties and one sensitive isolate (MR30) was used for comparision.All growth parameters of selected legumes were enhanced at 5% salt concentration as compare to control in CMR3,CMR13,MR5.MR11. A decline in all parameters was observed in isolate that does not show good cell surface components under salt stress. This shows the inhibition of cell surface properties under salt stress, affects the symbiotic performance of both parameters negatively. The present study concludes that under salt stress conditions, tolerant strains successfully overcome the stressful environmental conditions by maintaining the factors, essential for symbiosis in an ideal system.

Highlights

Soil salinity is one of the significant environmental problem facing agricultural production in many areas and affects approximately 7% of the world's total land area[1]
Saline behavior of many cultivated crops varies in different ways and depends on environmental factors like climatic conditions, soil properties, and the stage of growth[3] Rhizobia are more economically grows on many substrates[4,5,6] Rhizobial surface components contributes important parameter for deciding the perfect relation between host and plant for bringing about the infection that leads to nodulation and nitrogen fixation.7therefore cell surface components are the major property for understanding to identify stress-induced changes of Rhizobium to tolerate salt stress
Resistance of the isolates against NaCl was determined on streaking the cultures on yeast extract mannitol (YEM) with NaCl concentration ranging from 0%to 9.5%

Summary

Introduction

Soil salinity is one of the significant environmental problem facing agricultural production in many areas and affects approximately 7% of the world's total land area[1]. Saline behavior of many cultivated crops varies in different ways and depends on environmental factors like climatic conditions, soil properties, and the stage of growth[3] Rhizobia are more economically grows on many substrates[4,5,6] Rhizobial surface components contributes important parameter for deciding the perfect relation between host and plant for bringing about the infection that leads to nodulation and nitrogen fixation.7therefore cell surface components are the major property for understanding to identify stress-induced changes of Rhizobium to tolerate salt stress. These components include acidic expolysaccharide, neutral beta glucans, lipopolysaccharides and the elements responsible for motility of rhizobial cells

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