Effects of salinity on sulfur-dominated autotrophic denitrification microorganisms: Microbial community succession, key microorganisms and response mechanisms

Abstract

This study investigated the performance of sulfur-dominated autotrophic denitrification (SAD) process under four different salinities of 0 %, 0.5 %, 3 %, and 6 %, simulating groundwater, sewage, seawater, and industrial wastewater scenarios, respectively. In terms of removal efficiency, the nitrate removal rates at 0.5 % and 3 % salinities decreased by 5.8 % and 16.8 %, respectively, compared to 0 % salinity. At 6 % salinity, the efficiency fluctuated during long-term operation, ranging from 56.0 % to 89.9 % of the value at 0 % salinity. According to the succession of microbial communities across time and salinity gradients, the dominant denitrification functional microorganisms were ranked by their salinity-tolerance ability as low-tolerant bacteria (0 %–0.5 %: Simplicispira, Herpetosipin, and Thermomonas), medium-tolerant bacteria (0 %–3 %: Thiobacillus and Sulfurimonas), high-tolerant bacteria (3 %–6 %: Thiogranum, Xanthomarina, and Vicingus), and halophilic bacteria (6 %: Aequorivita). Additionally, Aequorivita and Thiogranum, as the top 2 genera under 6 % salinity, presented positive and negative correlations with nitrate removal efficiency. The molecular ecological network revealed that increased positive interactions of 58.7 % among dominant genera occurred at 6 % salinity, confirming an effective cooperation coping with the high-salinity stress. In addition, the microbial responses to high-salinity primarily exhibited 22 %–45 % decrease in protein content, a 15 % decrease in live cells proportion, and a comparable level of individual functional gene expression. The results indicate that the efficiency of SAD by high salinity is determined by biomass amount and individual microbial capacity. This study offered new sights for understanding microbial succession and relationships in response to saltwater with diverse origins and varying water qualities in SAD systems.