Effects of rosemary enriched diet on physiological parameters in common carp Cyprinus carpio L reared in cages culture

Abstract

The current study investigated at how rosemary leaf powder supplementation influenced the growth performance, carcass composition, hematological and biochemical parameters, stress hormones, and heat shock proteins of cage-reared common carp. Nine cylindrical cages of 0.2 m³ (radius = 25 cm, height = 100 cm) were randomly allocated to hold 54 juvenile common carp C. carpio (mean weight=18.18±0.08 g). Three groups of fish were fed a control diet (C) as well as diets containing 1.5% and 3% rosemary powder, respectively. The addition of 3% rosemary resulted in a substantial enhancement in growth parameters and feed consumption. The levels of WBC, Hb, MCHC, RBC, and Hct were significantly elevated by the addition of rosemary to experimental fish. A rosemary-supplemented diet resulted in higher levels of TG, urea, total protein, albumin, lipase, amylase, ferrum, and globulin. However, the addition of substantially reduced the levels of ALT cholesterol, creatinine, and glucose. The value of cortisol, T3, and T4 were considerably reduced with the addition of rosemary to diets of experimental fish. HSP70 and HSP90 levels in fish feed diets administered rosemary increased significantly as supplementation amount increased. According to the findings of this investigation, adding rosemary leaf powder, particularly at a concentration of 3%, in the diet improved growth parameters, hematological and biochemical parameters, and be able to reduce stress responses, thyroid hormone levels, and other stressors that fish exposed in cage culture system.