Effects of root flooding and stage of development on the growth and photosynthesis of field bean (Vicia faba L. minor)

Abstract

Field bean plants were subjected to flooding stress for 7 days, during two stages of development: at the vegetative phase (4-week-old seedlings) and at the generative phase (8-week-old plants). The height of plants, total area of leaves, the number of undamaged leaves, dry plant matter, chlorophyll content, ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBPCO) activity, the maximum quantum yield of PS2 photochemistry (Fv/Fm ratio), the photosynthesis rate (P N) and stomatal conductance (g s) were determined. A strong reduction in stem elongation and leaf area as well as in dry matter production was observed as a result of flooding. The responses from vegetative plants were greater than in generative plants. Waterlogging decreased chlorophyll a and b in leaves, notably at the vegetative stage, and persisted after cessation of flooding. After flooding, photosynthesis was strongly reduced and positively correlated with decreased stomatal conductance. Damage to the photosynthetic apparatus resulted in a lower Fv/Fm especially in young seedlings. In vegetative plants Fv/Fm quickly returned to the control levels after the soil was drained. The results show that an excess of water in the soil limits growth and injures the photosynthetic apparatus in field beans, but that the extent of the injury is strongly age dependent.