Abstract
The impact of OCI (Oryzacystatin I) expressing transgenic potato on Colorado potato beetle (CPB) larvae development was investigated. Transgenic potatoes, resistant to kanamycin and expressing the OCI cysteine protease inhibitor (PI), were obtained via Agrobacterium tumefaciens genetic transformation. Four independent transgenic lines were shown by molecular analysis to exhibit a high level of OCI expression. Larvae of CPB were independently reared on either transgenic or control potato leaves until the end of larval development. A significant impact of OCI transgenic potato on larval mortality was obtained, with up to 53% mortality recorded in larvae reared on transgenic leaves.
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