Abstract

Cancer is a major threat to the public health whether in developed or in developing countries. As the most common primary malignant tumor, the morbidity and mortality rate of lung cancer continues to rise in recent ten years worldwide. Chemotherapy is one of the main methods in the treatment of lung cancer, but this is hampered by chemotherapy drug resistance, especially MDR. As a component of the 60S large ribosomal subunit, ribosomal protein L39-L gene was reported to be expressed specifically in the human testis and human cancer samples of various tissue origins. Total RNA of cultured drug-resistant and susceptible A549 cells was isolated, and real time quantitative RT-PCR were used to indicate the transcribe difference between amycin resistant and susceptible strain of A549 cells. Viability assay were used to show the amycin resistance difference in RPL39-L transfected A549 cell line than control vector and null-transfected A549 cell line. The ribosomal protein L39-L transcription level was 8.2 times higher in drug-resistant human lung cancer A549 cell line than in susceptible A549 cell line by quantitative RT-PCR analysis. The ribosomal protein L39-L transfected cells showed enhanced drug resistance compared to plasmid vector-transfected or null-transfected cells as determined by methyl tritiated thymidine (3H-TdR) incorporation. The ribosomal protein L39-L gene may have effects on the drug resistance mechanism of lung cancer A549 cells.

Highlights

  • The international agency for research on cancer (IARC) released the world cancer report showed that cancer has a major threat to the public health whether in developed or in developing countries

  • The drug-resistant human lung cancer A549 cell line has been selected from a susceptible A549 cell line, and the resistance has been maintained by treatment with amycin at LC50 of each generation

  • The results suggested that RPL39-L expression was up-regulated in drug-resistant human lung cancer A549 cell line (Figure 1)

Read more

Summary

Introduction

The international agency for research on cancer (IARC) released the world cancer report showed that cancer has a major threat to the public health whether in developed or in developing countries. Lung cancer is the most common lung primary malignant tumor, the morbidity and mortality rate continues to rise in recent ten years worldwide, lung cancer has become one of the major diseases affecting human health (Villanueva, 2014). There have been many research studies done on the lung cancer multidrug resistance mechanism all over the world in recent years. As the most common primary malignant tumor, the morbidity and mortality rate of lung cancer continues to rise in recent ten years worldwide. Results: The ribosomal protein L39-L transcription level was 8.2 times higher in drug-resistant human lung cancer A549 cell line than in susceptible A549 cell line by quantitative RT-PCR analysis. Conclusions and Implications for Practice: The ribosomal protein L39-L gene may have effects on the drug resistance mechanism of lung cancer A549 cells

Methods
Results
Conclusion
Full Text
Published version (Free)

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call