Abstract
Abstract Objectives To investigate the possible protective effects of resveratrol against oxidative testicular damage due to scattered radiation during pelvic ionizing radiation exposure in rats. Methods Rats were divided into 5 groups; control, radiation, and radiation + resveratrol therapy in early and late periods. Under anesthesia, 20 Gy ionizing radiation was applied to prostatic region. Resveratrol was administered (10 mg/kg/day) orally before ionizing radiation exposure. Animals were decapitated at the end of 1st and 10th weeks. Biochemical markers of oxidative stress; caspase-3 and sirtuin-1 protein expressions; testosterone levels were evaluated, histological examinations were performed. Results Significant increases in malondialdehyde, 8-hydroxy-deoxyguanosine levels, myeloperoxidase, and caspase-3 activities were observed after ionizing radiation exposure, also superoxide dismutase and glutathione activities were significantly decreased. Radiotherapy increased caspase-3 and decreased sirtuin-1 protein expressions. Resveratrol treatment significantly reversed these parameters and also reversed the decrease in testosterone levels back to control levels in late period. Conclusion Resveratrol showed antioxidant and sirtuin-activating properties against oxidative damage caused by scattered radiation to testis and provided hormonal protection. These results suggest that resveratrol may be an alternative protective agent on testicular tissues against the effects of scattered pelvic radiation.
Highlights
The mechanism of action of radiotherapy is based on two principles
These results suggest that resveratrol may be an alternative protective agent on testicular tissues against the effects of scattered pelvic radiation
Samples resolved by 4–12% sodium dodecyl sulphate–polyacrylamide gel electrophoresis were transferred to polyvinylidene fluoride (PVDF) membrane, which was blocked with bovine serum albumin
Summary
The mechanism of action of radiotherapy is based on two principles. The radiation applied to living tissue is divided into electrically charged ions, and when these ions pass through the cells, accumulated energy directly damages the cells. While radiation to targeted organ destroys the tumor with high amounts of generated ROS, the scattered beam, at a lower density, causes damage to surrounding tissues, along with further creation of ROS. This scattered radiation phenomenon constitutes the mainstay of emergence of undesirable radiotherapy effects [1]. Fast-dividing cells are generally more susceptible to radiation than slowdividing cells [2] Many of these side effects impair the quality of life of patients undergoing radiotherapy for cancer treatment and reduce the patient’s resistance to treatment [3, 4]
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