Effects of Resistance Exercise on Kidney Antioxidant Enzymes of Rats Treated with Doxorubicin

Abstract

The aim of this study was to investigate the effects of the anti‐cancer drug doxorubicin (DOX) and resistance exercise training on kidney antioxidant enzyme expression. Male rats were assigned to a resistance training (RR) or sedentary (SS) group for the first 10 weeks of the study. RR were housed in specialized resistance training cages, and SS were house in standard cages. After 10 weeks, animals received either weekly 3 mg/kg intraperitoneal injections of DOX over 4 weeks (12 mg/kg total, SSD, RRD) or an equivalent doses of 0.9% saline as control and continued with the activity treatments (SSS, RRS). Five days after the last DOX injection, kidneys were collected, and expression of the antioxidants catalase (CAT), superoxide dismutase 1 (SOD1), and glutathione peroxidase (GPx) was analyzed using Western blotting. Significant activity and drug main effects were observed for CAT expression (p=0.02 and p=0.0002, respectively) with SSD expressing significantly lower CAT than all other groups (p<0.05). With SOD1 expression, a significant activity effect (p=0.0088), drug effect (p<0.0001), and activity × drug interaction (p=0.0347) was observed. No significant main effects or interactions were observed for GPx, but there was a trend toward an activity effect (p=0.0706). Although more work is needed, the results of this study are promising, and we conclude that resistance training prior to and during DOX treatment may help to minimize renal damage by upregulating antioxidant enzyme expression.This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.