Abstract
Objective To indentify effects of the replication-deficient recombinant adenovirus carrying cytochrome P450 (CYP2B6) gene on human prostate cancer cell line PC-3.Methods The human prostate cancer cell line PC-3 cells were infected with the replication-deficient recombinant adenovirus carrying CYP2B6 gene by the MOI of 200 VP/cell.The expression level of CYP2B6 mRNA and protein was detected by using real-time quantitative polymerase chain reaction (Real-time PCR) and Western blotting respectively.Results Compared to the negative control,after infection of the replication-deficient recombinant adenovirus carrying CYP2B6 gene,PC-3 cells expressed higher level of CYP2B6 mRNA (8.12 ± 2.41 vs.0.92 ±0.19,P ＜0.05) and CYP2B6 protein (0.82 ±0.23 vs.0.12 ±0.06,P ＜0.05).No significant difference was observed between the ZERO group (PC-3 cells not infected with adenovirus) and negative control in CYP2B6 mRNA expression level (0.78 ±0.27 vs.0.92 ±0.19,P ＞0.05) and protein expression level (0.14 ± 0.03 vs.0.12 ± 0.06,P ＞ 0.05).Conclusion The replication-deficient recombinant adenovirus carrying CYP2B6 gene can infect human prostate cancer cell line PC-3 effectively,and these PC-3 cells can express CYP2B6 mRNA and CYP2B6 protein effectively. Key words: Prostate cancer; Cytochrome P450 gene; Replication-deficient adenoviral
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