Effects of replacement of dietary fish oil with soybean oil on growth performance and tissue fatty acid composition in marine herbivorous teleost Siganus canaliculatus

Abstract

Fish oil (FO) substitution has been studied in many marine carnivorous fish, but seldom in marine herbivorous or omnivorous species. To evaluate the feasibility of using soybean oil (SO) as a dietary lipid and confirm its capability of converting C18 polyunsaturated fatty acid (PUFA) into long chain polyunsaturated fatty acid (LC-PUFA) in the marine herbivorous teleost Siganus canaliculatus, juvenile fish were fed with four formulated diets differing in lipid composition, with SO accounting for 0.76% (SO0), 23% (SO23), 45% (SO45) and 67% (SO67) of total dietary lipid respectively. After feeding for 8 weeks, growth performance including weight gain, specific growth rate, feed conversion ratio and protein efficiency rate were better in the SO23 and, especially, SO45 groups than in the SO0 and SO67 groups (P < 0.05). Tissue fatty acid compositions were affected by diet, with the liver contents of eicosapentaenoic (EPA), docosapentaenoic (DPA), docosahexaenoic (DHA) acids and total n-3 PUFA displaying parallel changes with the corresponding dietary fatty acids. While the muscle contents of EPA, DPA and total n-3 PUFA between SO0 and SO23 groups, and the liver contents of arachidonic acid (ARA) and 20:4n-3, as well as the muscle content of 20:3n-6 between SO0 and SO45 groups showed no difference, confirming the biosynthesis of LC-PUFA from C18 precursors in vivo as the contents of corresponding fatty acids in diets SO23/SO45 were much lower than those in diet SO0 (P < 0.05). The results indicate that SO may be a suitable dietary lipid source for S. canaliculatus, and can replace up to 67% or 45% of total dietary FO without negatively compromising growth performance or nutritional quality of fish respectively. Moreover, the study increases our knowledge of FO substitution in marine herbivorous fish.