Abstract
Effects of repeated applications of chlorpyrifos on its persistence and soil microbial functional diversity were studied under laboratory conditions. The results showed that the degradation rate of chlorpyrifos increased whereas its inhibitory effect on soil microbial communities gradually decreased with application frequency of chlorpyrifos. A bacterial strain DSP capable of utilizing chlorpyrifos as a sole source of carbon and energy was isolated 21 days after the third chlorpyrifos application, which indicated that the capability of soil microorganism for degrading chlorpyrifos was formed during the experiment. It could be concluded that repeated applications of chlorpyrifos had no lasting impact on soil health.
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