Effects of renal denervation on left ventricular remodeling and cardiac oxidative stress after myocardial infarction in canine

Abstract

Objective To investigate the effects of renal denervation (RDN) on left ventricular remodeling and cardiac oxidative stress due to myocardial infarction (MI) in canine. Methods Anterior myocardial infarction was produced by gelatin sponge embolization of the left anterior descending artery. Eighteen canines were randomly divided into three groups. Sham group (renal arteriography performed a week after coronary angiograph, n=6), MI group (renal arteriography performed a week after MI, n=6), and RDN group (RDN performed a week after MI, n=6). Four weeks post-MI, left ventricular function was measured by hemodynamics. Echocardiography examination was administered to identify left ventricular end-diastolic dimension (LVEDD), left ventricular end systolic diameter (LVESD), fraction shortening (FS), and left ventricular ejection fraction (LVEF). Anti-superoxide anion free radical (ASAFR) activity was detected by colorimetry. Thiobarbituric acid method was used to measure the concentration of malonaldehyde (MDA). Results Compared to Sham group, the LVEF, FS and left ventricular systolic pressure (LVSP) in MI group and RDN group were decreased obviously (all P 0.05). There were no significant difference in heart rate (HR) among three groups (P>0.05). Although the levels of MDA, ASAFR, and p47phox mRNA were higher in MI group and RDN group, compared to the Sham group (all P<0.05), they were all significantly decreased in RDN group compared to the MI group (all P<0.05). Conclusions RDN was able to improve heart function and myocardial remodeling and inhibit cardiac oxidative stress. Key words: Sympathectomy; Kidney/IR; Myocardial infarction/CO/SU; Ventricular remodeling; Oxidative stress; Myocardium/PA