Abstract
Objective To observe the effects of reduced glutathione on pulmonary apoptosis induced by different tidal volume mechanical ventilation in rats.Methods Thirty SD rats were randomly divided into five groups:control group(group A,n=6),normal ventilation group(group B,n=6),hyperventilation group(group C,n=6),normal ventilation+reduced glutathione group(group D,n=6),hyperventilation+reduced glutathione group(group E,n=6).The Nuclear factor-κΒ(NF-kB)expression in pulmonary endothelium was assessed by histochemical staining;the methods of ELISA was used to determined the level of tumor necrosis factor alpha (TNF-α).Apoptosis was investigated by TdT-mediated dUTP nick end labeling.Results The expression of the NF-κB,TNF-α was significantly higher in group C than in group A and group B,group D,group E.In group C pulmonary apoptosis were obviously than in group D and E (P＜0.01).Conclusions Reduced glutathione can decrease pulmonary apoptosis ventilation-induced lung injury. Key words: Glutathione; Mechanical ventilation; Tidal volume; Apoptosis; Animal testing alternative; Rats
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