Effects of Recombinant Adenovirus-p53 Combined with Oxaliplatin on Growth Inhibition of Human Gastric Cancer Cell Line SGC-7901

Abstract

Objective To observe the expression of exogenous p53 gene in gastric cancer cells and its effects on the growth of tumor cells;and to investigate the effects of adenovirusmediated p53 gene on chemosensitivity of human gastric cell and the value of gene therapy combined with chemotherapy.Methods Toinvestigate the effects of recombinant adenovirus-p53(rAd-p53) and oxaliplatin(OXA) alone and combined on the gastric cancer cell line SGC-7901 for different times,CCK-8 assay was used to examine the suppressive rate of cell growth dealt;p53 protein expression was detected by immunohistochemistry assay;and protein caspase-3 expression in cell was induced by different drugs alone and in combination by flow cytometry.Result When rAd-p53 and OXA was alone used to treat the gastric cancer cell line SGC-7901,with an increase in drug concentration and treated time,the cell growth inhibition rate gradually increased;when rAd-p53 and OXA was combined to treat the gastric cancer cell line SGC-7901 for 48 h,the cell growth inhibition rate gradually increased at the lowest concentration.When rAd-p53(5×107,5×108,5×109vp/ml) and OXA(6.25μg/ml) was combined to treat the gastric cancer cell line SGC-7901 for 48h,compared with the control group,the content of caspase-3 protein in gastric cancer cell gradually increased,but p53 protein expression didn't increase.Conclusion The proliferation of SGC-7901 could be inhibited by rAd-p53 OXA alone,but when rAd-p53 was combined with OXA,the proliferation of cells was higher than that used alone.The combination of rAd-p53 and OXA induced cell apoptosis through mitochondrial pathway to activate downstream of caspase-3.