Abstract
To explore the effects of receptor interacting protein (RIP)140 gene over-expression upon the migration and proliferation of neuroblastoma cells in vitro. The N2a RIP140 over-expression model (N2a-rip140) was constructed by lipofection and validated by real-time polymerase chain reaction (PCR) and Western blot. The migration and proliferation capabilities were compared between N2a-rip140 and its parents by Transwell chamber and CCK-8. The N2a RIP140 over-expression model (N2a-rip140) was successfully constructed. Compared to N2a group, the RIP140 mRNA and protein expression levels of N2a-rip140 were remarkably up-regulated. Transwell assay showed that the over-expression of RIP140 inhibited N2a cell migration ((70 ± 20) vs (5 ± 4) cells, P < 0.05). CCK-8 assay showed that the proliferation rates of N2a group at 24, 48, 72 h were 1.567 ± 0.107, 3.018 ± 0.212 and 4.112 ± 0.221 respectively. And those of N2a-rip140 group were 1.561 ± 0.281, 2.232 ± 0.235 and 4.025 ± 0.217 respectively. No significant difference existed in proliferation rates at different timepoints among N2a, N2a-M and N2a-rip140 groups (all P > 0.05). RIP140 over-expression effectively inhibits N2a cell migration. However it has no significant effect on the proliferation of N2a cells.
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