Abstract
AIM: To investigate the effect of radix salviae miltiorrhizae (RSM) on the changes of [Ca]i induced by endothelin-1 (ET-1) in hepatic stellate cells (HSCs). METHODS: After preparation of RSM cream, the effects of RSM on ET-1-induced changes of HSCs [Ca]i were observed by laser scanning confocal microscopy. RESULTS: In normal buffer (including Ca, buffer A), the fluorescence intensity was enhanced accordingly with the increase of ET-1 concentration. The cumulative-response curve showed EC50 was 1.1×10 mol/L. After incubation of HSCs with ET-1 in buffer A and buffer B (absence of extracellular calcium, EGTA), the duration of calcium peak had significant difference (165.2 ± 10.1 s vs 91.0 ± 7.2 s, P 0.05). In the cells pre-incubated with RSM, KCl-induced elevation of [Ca]i was decreased, and the calcium peak value (78.0% ± 6.1% → 26.3% ± 1.2%, P < 0.01) and duration of calcium wave (70.8 ± 10.4 s → 15.9 ± 5.1 s, P < 0.01) were decreased significantly. CONCLUSION: RSM inhibits ET-1-induced depletion of intracellular calcium, which has no correlations with the influx of extracellular calcium. RSM can also inhibit KCl-induced influx of calcium, indicating its characteristic of blocking voltage-operated Ca channel.
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