Effects of quercetin on LPS-induced disseminated intravascular coagulation (DIC) in rabbits

Abstract

IntroductionQuercetin is widely distributed in plants and has been reported to have effects of anti-inflammation and anti-thrombosis. In this study, we evaluated the protective effect of quercetin on LPS-induced experimental DIC in rabbits, and tried to clarify its mechanism against DIC. Materials and MethodsLPS-induced DIC model in rabbits was established through continuous infusion of 100 ug·kg-1·h-1 LPS for a period of 6h. Six groups were divided: quercetin-treated groups (0.5, 1.0, and 2.0mg·kg-1·h-1, respectively), LPS-control group, heparin-control group (100IU·kg-1·h-1), and saline-control group. APTT, PT, and plasma FIB level were measured, the plasma levels of ALT, BUN, and TNF-α were detected, and the activity of Protein C and ATIII was recorded. ResultsA continuous injection of LPS induced a gradual impairment of hemostatic parameters, a rise in plasma level of TNF-α, and damage in renal and hepatic function. The intravenous administration of quercetin significantly attenuated the increase of APTT, PT, ALT, BUN, and TNF-α, and the decrease of plasma FIB level and activity of Protein C and ATIII. ConclusionQuercetin may have a protective effect against LPS-induced DIC in rabbits through anti-inflammation and anticoagulation.