Abstract
Polychlorinated biphenyls (PCBs) are widespread persistent residual environmental pollutants, which affect seriously the growth and reproductive alterations in humans and animals. Aroclor 1254 is a commercial mixture of PCBs. Quercetin is a flavonoid, which acts on estrogen receptors and causes the development of estrogen-related diseases. In this paper, the primary cultured endometrial cells in the pregnant rats were isolated and Aroclor 1254 was used to induce the injured endometrial cells model. The cells were treated with gradient quercetin, the viability of the endometrial cells, the expressions of CYP450, the contents of TNF-α, IL-6, estradiol (E2), and progesterone (P4) were measured. It showed that the viability of the cultured endometrial cells, the expression of CYP1A1 and CYP2B1, and the contents of TNF-α, E2, and IL-6 in the injured endometrial cells increased with the treatment of quercetin. It shows that quercetin has protective effect on the injured endometrial cells in the pregnant rats, this provide a basis on herbal medicine protection for animal reproductive diseases caused by environmental endocrine disruptors.
Highlights
Polychlorinated biphenyls (PCBs) are widespread persistent residual environmental pollutants, which have been widely used for various industrial applications [1]
PCB can result in an imbalance in the cellular oxidative stress/antioxidant status and cause cell injury; oxidative stress can play a critical role in observed PCB mediated endothelial cell dysfunction
The expression of CYP1A1 and CYP2B1 increased as the Aroclor 1254 dose increased, the highest amount of CYP1A1 and CYP2B1 expression was in 1 μg/mL group, and the expression decreased with treatment 10 μg/mL Aroclor 1254 group (Figure 2)
Summary
Polychlorinated biphenyls (PCBs) are widespread persistent residual environmental pollutants, which have been widely used for various industrial applications [1]. PCBs affect seriously the growth and reproductive alterations in humans and animals [2,3,4]. PCBs convert into hydroxy-PCBs in the liver [5, 6]; hydroxy-PCBs produced estrogen and thyroid interference effects in the body and caused serious influence to reproductive functions [7]. Higher dosages of PCBs adversely affect fertilization and cause degeneration of oocytes and abnormality in the early mouse embryo [8]. Crinnion [9] and Fadhel et al [10] found that most of the PCBs congeners could induce the metabolic enzymes in vivo through the aryl hydrocarbon (Ah) receptor signal transduction pathway
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